Surface-active extracts from plants rich in saponins – effect on lipid mono- and bilayers

The aqueous extracts of the seeds oat ( Avena sativa L.), horse chestnut Aesculus hippocastanum L . ), soybean Glycine max cowherb Vaccaria hispanica [P. Mill.] Rauschert) and quinoa Chenopodium Willd.), roots soapwort Saponaria officinalis L.) without any preservatives were characterized in terms their surface tension, compression (dilational) rheology, foamability foam stability. saponin content was determined using UPLC-MS interaction with model lipid monolayers consisting dipalmitoylphosphatidylcholine (DPPC)/cholesterol Ceramide AP/stearic acid/cholesterol analyzed by pressure relaxation, elasticity neutron reflectometry (NR). composition chosen to mimic cell membrane keratinocytes – major constituents human deeper skin layers, intercellular lipids (“mortar”) “bricks mortar” outermost layer epidermis stratum corneum ). Bilayers DPPC/cholesterol additionally dynamic light scattering (DLS) NR. shown be much less abundant saponins as compared cowherb, chestnut, or quinoa, showed limited foaming abilities. They did not affect significantly mono- bilayers mimicking outer either. extract affected both membranes highest extent, yet solubilizing lipids.